TABLE 1.
Various synthetic peptides generated to binding, translocation, and enzymatic domains of ETA and rabbit group designation for the immunization protocol
| Rabbit group | Domain of ETA | Peptide designation | Amino acid residues corresponding to ETA sequencea |
|---|---|---|---|
| I | Binding (Ia) | Peptide 1 | 99–110 |
| Peptide 2 | 201–215 | ||
| Peptide 7 | 143–157 | ||
| Peptide 10 | 26–41 | ||
| II | Translocation (II) | Peptide 3 | 297–310 |
| Peptide 8 | 352–362 | ||
| Peptide 9 | 289–333 | ||
| IIIa | Enzymatic (III) | Peptide 4 | 508–520 |
| Peptide 5 | 598–618 | ||
| Peptide 6 | 626–638 | ||
| IIIb | Enzymatic (III) | Peptide 11 | 610–638 |
| IV | Holotoxin | 1–638b | |
| Vc | KLH |
a
Peptides were synthesized to match regions within the three different domains of ETA which have high surface probability.
b
Amino acid residues 1 through 638 represent the ETA amino acid sequence, including the leader peptide sequence, which corresponds to aa 1 to 25.
c
Group of two rabbits immunized with KLH (the carrier protein used in peptide conjugation).