Fig. 1.
The formation of destruction complex condensates depends on the β-catenin accumulation of high concentrations in APC wild-type cells. A Representative live-cell images of β-catenin puncta formation after transiently expressing β-catenin at different protein concentrations in CTNNB1 KO HEK293T cells. B–C Representative live-cell images of β-catenin (green) and Axin (red) expression after transfection of β-catenin and mutant Axin (Δ209-464aa, Δ497-679aa) B or β-catenin and WT Axin C in CTNNB1 KO HEK293T cells. D–E Immunofluorescent staining of Axin (red), β-catenin (green), and nuclear (DAPI, blue) in FFPE tissues from CRC patients D or adjacent normal intestinal epithelial tissues, and APCMin/+ mouse adenocarcinoma tissues E or intestinal epithelial tissue of wild-type mice. F Left: immunofluorescent staining of Axin (red), β-catenin (green), and nuclear (DAPI, blue) in HCT15 cells. Right: quantitative determination of co-locatable protein aggregate points in 5 cells