Fig. 2.

Truncated APC mutants maintain the presence of the destruction complex condensates. A Confocal images of intracellular truncation mutant EGFP-tagged APC protein in SW480. The truncated sites are aa1450 and aa876, respectively, named APC^R1450* and APC ^R876*. B Left: representative images of the APC^R1450* and APC ^R876* cells treated with 5% 1,6-hexanediol (1,6-HD) for 1 min in CRC cells. Right: quantification of the number of APC^R1450* and APC ^R876* puncta per cell at the indicated times with 5% 1,6-HD. n = 8; *, p < 0.05; ***, p < 0.001. C Representative live-cell images of puncta formation transfected with Axin (red) and APC^R1450* (left, green) or APC ^R876* (right, green) in CRC cells. D FRAP analysis of Axin and truncated APC^R1450* (left) or APC ^R876* (right) puncta in CRC cells. Curves show the average time course of normalized fluorescence intensity. n = 5 cells. E Confocal images of live CRC cells expressing Axin (red), APC^R1450* (up, green), or APC ^R876* (down, green) and β-catenin (yellow) puncta. The arrow indicates representative colocalization puncta. F Representative images of live SW480 cells transfected with Axin-IDR (aa209-679) (red) and APC^R1450* (left, green) or APC ^R876* (right, green) proteins