Fig. 4.

Multiplex immunofluorescence staining of CRC patient and APC^Min/+ mouse adenoma tissues. A–D Left: multiplex immunofluorescence staining of Axin (red), β-catenin(white), CK1α (green), GSK3β (green), and nuclear (DAPI, blue) in the intestinal epithelial tissues of APC^Min/+ mouse or wild-type mice (A–B), CRC patients and adjacent normal tissues (C–D). The arrows indicate representative the colocalization of Axin and CK1α or Axin and GSK3β regions. Right: quantitative determination of the correlation of colocalization between different proteins by Mander’s overlap coefficient (MOC) analysis in ten high power fields. Error bars indicate the SEM. *, p < 0.05; **, p < 0.001