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. 2024 Jan 27;81(1):57. doi: 10.1007/s00018-023-05068-0

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — Axin is involved in the β-catenin nuclear translocation and transcriptional function as a droplet. A Confocal images of intracellular Axin (red) and β-catenin (green) in CRC cells. Line four: representative images of 3D reconstitution using by Imaris software. Line five: section drawing of 3D reconstructed confocal images by Imaris software. The tool, Clipping Plane, which allows to re-slice in any chosen rotation (https://imaris.oxinst.com/), uncovers hidden regions by re-slicing the image data in a different orientation. B Representative images of Axin (red) and nuclear (DAPI, blue) expression after transfection of NLS-mutated Axin in HEK293T and SW480. C Cells were treated with or without Leptomycin B (LMB, 200 nM, 24 h). Nuclear and cytoplasmic protein extraction experiment was further performed to measure the cellular localization of Axin in SW480 cells after transfection of NLS-mutated Axin. D The mRNA levels of Wnt pathway-specific downstream target gene (Axin2) were examined by RT-PCR in SW480 cells expressing wild-type Axin or NLS-mutated Axin. Error bars, mean ± SD; **, p < 0.01. E Analysis of TOP/FOP reporter activity in SW480 cells transfected with wild-type Axin or NLS-mutated Axin. Error bars, mean ± SD; **, p < 0.01. F Representative images of β-catenin (green) and nuclear (DAPI, blue) expression after transfection of β-catenin in Axin KO SW480 or wild-type SW480 cells. G Representative fluorescence images of live SW480 and HEK293T after transiently expressing EGFP-tagged Med1-IDR and pScarlet-tagged Axin