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Amplification of the gtfB-gtfC region of gbpA mutant S. mutans UA130 rough and smooth colonies. Long-range PCR and electrophoresis were carried out as described in the text. The sizes (in kilobases) of StyI-digested lambda DNA (lanes M) are indicated in the left margin. Lanes 2 to 9, colonies recovered from four rats (A2 to A5) 36 days postinoculation; 2, A2-1 smooth; 3, A2-1 rough; 4, A3-1 smooth; 5, A3-1 rough; 6, A4-1 smooth; 7, A4-1 rough; 8, A5-1 smooth; 9, A5-1 rough.
