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. 2024 Jan 26;15:780. doi: 10.1038/s41467-024-45141-1

Fig. 1. Identification of UBR5 as a positive regulator of MDA5 signaling.

Fig. 1

a Graphical illustration of the experimental flow (Created by Figdraw., Figdraw export ID: PUWAU5be43). Individual E3 knockout 2fTGH-ISRE-Luc cells were transfected with (b) high molecular weight poly (I:C) to stimulate MDA5, c immunostimulatory DNA (ISD) to activate cGAS, or (d) treated (no transfection) with recombinant human IFN-β to trigger JAK-STAT1/2 signaling for 12 h. The luciferase activity in each cell line is expressed as the average fold change over WT. The blue/red/gray dots are the knockouts in which the luciferase activity is higher/lower/ not different, than that in WT (black dot). e–g The luciferase results for select E3 knockouts and positive controls. Data are presented as mean ± S.E.M, ordinary one-way ANOVA with Dunnett’s test; n = 3 biological independent experiments; p values are assigned as -log(P) in b–d; ****p < 0.0001 vs WT in e. Multiplicity adjusted p values are presented. Mock: lipofectamine in b, c, e, f or sterile water in d, g only. Source data are provided as a Source Data file.