Fig. 4. UBR5 is crucial for the induction of innate immune response and control of RNA virus pathogenesis in mice.

a The immunoblots of UBR5 in various tissues of age- and sex-matched littermates. Black triangle indicates the right band size. The survival curves of littermates infected with (b) 100 or (c) 1,000 plaque forming units (PFU) of EMCV intraperitoneally. In b, n = 16 for Ubr5^WT and 17 for Ubr5^iKO, p = 0.038 (Log-Rank test); in c, n = 14 for Ubr5^WT and 10 for Ubr5^iKO, p = 0.030 (Log-Rank test). Quantification of (d) EMCV RNA in the whole blood cells by qRT-PCR, e viremia and (f) viral loads in hearts by a plaque forming assay, in the mice infected with 100 PFU of EMCV. In d–f, n = 6 mice, mean ± S.E.M., two-tailed, unpaired non–parametric Mann–Whitney U test; *p = 0.0411 (d), *p = 0.0152 (e), *p = 0.0411 (f). g, h Quantification of the serum type I IFN and cytokine concentrations by ELISA in the mice infected with 1 × 10⁷ PFU of EMCV intravenously. n = 7 mice/group, mean ± S.E.M., two-tailed Student’s t test; ***p = 0.0009 for IFN-β, *p = 0.0221 for IFN-α (g); *p = 0.0259 for TNF-α, **p = 0.0055 for CXCL10, **p = 0.0034 for MIP−1α, **p = 0.0049 for MIP−1β (h). i The survival curves of age- and sex-matched littermates infected with 1 × 10⁷ PFU of VSV intravenously. n = 11 mice/group, p = 0.03 (Log-Rank test). j The arbitrary morbidity score of VSV-infected animals. n = 6 mice/group, mean ± S.E.M., two-tailed Student’s t test; *p = 0.0438, *p = 0.0438 in sequence. Adjusted p values are presented. All the mice used in this study were 8 weeks old. Source data are provided as a Source Data file.