FIG. 1.

Map of CAP60. (A) Restriction enzyme map. Overlapping subclones of pYCC107 were diagrammed. pYCC120 is the reconstituted genomic clone. Plasmids were transformed into cap60-17FO7, and the capsular phenotypes of the resulting transformants were as indicated (+, present; −, absent). Open and hatched boxes represent the coding regions of CEL1 and CAP60, respectively. B, BamHI; D, NdeI; N, NcoI; P, PstI; S, SmaI; X, XhoI; V, EcoRV; Xb, XbaI. (B) Transcriptional direction of CAP60 and CEL1. Arrows indicate the direction of transcription. Triangles represent introns.