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. 2024 Jan 22;12(1):108. doi: 10.3390/vaccines12010108

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© 2024 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Diagram of WHEP vector and SDS-PAGE results of recombinant pertussis antigens. Recombinant pertussis antigens were produced by (a) WHEP vector. WHEP is a chaperna domain of human origin, derived from glutamyl prolyl tRNA synthetase. Hexa-histidine tag inserted to the vector for Ni-affinity purification. Purified antigens were indicated by (b) SDS-PAGE analysis of raPs. TEV, site-specific TEV protease recognition sequence; EK, site-specific enterokinase recognition sequence; MCS, multi-cloning site.