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Inhibition of biotinylated heparin binding to immobilized MBP-Fim2 by fimbriae. Biotinylated heparin was preincubated with a serial dilution of fimbriae for 1 h at 37°C. The suspension was then added to immobilized MBP-Fim2, and the amount of bound heparin was detected with a streptavidin-peroxidase conjugate. The assay was carried out at least three times, and a representative result is shown.
