Figure 5.

H₂O₂-induced DNA damage was prevented at lower concentrations of DHOLE. Representative images of the Comet assay after pre-treatment with DHOLE in the absence or presence of 250 μΜ H₂O₂ at EA.hy926 cells (A–F) and at C2C12 cells (G–L). (A,G): untreated cells (control), (B,H): cells treated with 250 μΜ H₂O₂ for 1 h, (C,I): treated cells with 10 μg/mL and 5 μg/mL, respectively, of DHOLE for 24 h, (D,J): pre-treated cells with 10 μg/mL and 5 μg/mL, respectively, of the extract for 23 h followed by incubation with 250 μΜ H₂O₂ for 1 h, (E,K): treated cells with 40 μg/mL and 10 μg/mL, respectively, of DHOLE for 24 h, (F,L): pre-treated cells with 40 μg/mL and 10 μg/mL, respectively, of the extract for 23 h followed by incubation with 250 μΜ H₂O₂ for 1 h.