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. 2024 Jan 19;16(2):305. doi: 10.3390/nu16020305

Figure 4.

Figure 4

Expression of genes associated with mitochondrial oxidative capacity in McPc-treated C2C12 myotubes. (A) Expression of mRNA-encoding transcription factors and coactivators related to mitochondrial oxidative capacity in C2C12 myotubes incubated with McPc for 24 h. (B) Glycolysis-related gene expression. (C) Fatty-acid-oxidation-related gene expression. (D) Oxidative phosphorylation (OXPHOS)-associated gene expression. (E) Levels of OXPHOS complex proteins (CI: NDUFB8, CII: SDHB, CIII: UQCRC2, CV: ATP5A) in C2C12 myotubes treated with McPc for 24 h. (F) Protein levels in C2C12 myotubes treated with McPc for 24 h. (G) Mitochondrial content of 100 μg/mL McPc-treated C2C12 myotubes measured using the MitoTracker staining assay. The density of MitoTracker Deep Red FM fluorescent was evaluated using a microplate reader (λex 644 nm and λem 665 nm). The values represent mean ± SD; * p < 0.05, ** p < 0.01, and *** p < 0.001. One-way ANOVA Tukey’s post hoc test was conducted for multiple comparisons and two-tailed unpaired Student’s t-test was conducted for single comparisons.