Table 1.

Primers used for site-directed mutagenesis.

Primer Designation	Primer Sequence (5′ -> 3′) 1
gH A651T	C GTG GTG ACC TCC GTC GTG G
gH I662A	CG GCC ATC ACC GTG GGG GCC CTG TAC GCC CTA TTC
gH I662G	CG GCC ATC ACC GTG GGG GGC CTG TAC GCC CTA TTC
gH I662R	CG GCC ATC ACC GTG GGG CGC CTG TAC GCC CTA TTC
gH I662S	CG GCC ATC ACC GTG GGG AGC CTG TAC GCC CTA TTC
gH I662T	CG GCC ATC ACC GTG GGG ACC CTG TAC GCC CTA TTC
gH I662Y	CG GCC ATC ACC GTG GGG TAC CTG TAC GCC CTA TTC
gH I662C	CG GCC ATC ACC GTG GGG TGC CTG TAC GCC CTA TTC
gH I662V	CG GCC ATC ACC GTG GGG GTC CTG TAC GCC CTA TTC

¹ Nucleotides exchanged are underlined; only the forward primers are given.