FIG. 1.

SDS-PAGE analysis of OMP26 and two other proteins with higher molecular masses. Proteins were separated on a 12% acrylamide-BIS gel by SDS-PAGE. Lane 1, molecular mass standards (molecular masses shown in kilodaltons on the left). Lanes 2 and 3, the protein samples were mixed with SDS reducing buffer but were not boiled. Lane 2, purified OMP26; lane 3, mixed protein sample of the three main proteins isolated in this molecular weight range. Lanes 4 and 5, same samples as in lanes 2 and 3, respectively, but heated for 30 min at 100°C in SDS reducing buffer containing β-mercaptoethanol.