TABLE 2.
Comparison of OMP26-specific antibodies in serum and BAL following immunization with either OMP26 or killed bacteria from H. influenzae strains
| Groupb | n | ELISA titera of antibody to OMP26a
|
|||||
|---|---|---|---|---|---|---|---|
| Serum
|
BAL
|
||||||
| IgG | IgA | IgM | IgG | IgA | IgM | ||
| Nonimmune | 7 | NDd | ND | 10.5 ± 1.6 | ND | ND | ND |
| Immunized | |||||||
| OMP26 | |||||||
| 10 μg | 5 | 1,209 ± 255e | 936 ± 161e | 30.5 ± 4e | 4.3 ± 1.0e | 6.1 ± 1.9e | ND |
| 40 μg | 5 | 2,806 ± 405e | 2,440 ± 410e | 142.5 ± 30.6e | 8.3 ± 1.9e | 29.1 ± 7.9e | ND |
| Killed bacteriac | |||||||
| NTHI-Ic | 4 | 58 ± 17e | 37 ± 4e | 39.1 ± 0.8e | 0.5 ± 0.1 | 1.2 ± 0.5 | ND |
| NTHI-IIc | 4 | 22 ± 4e | 26 ± 5e | 39.3 ± 5.0e | 0.6 ± 0.1 | 0.4 ± 0.1 | ND |
| HI-CDc | 4 | 18 ± 1e | 20 ± 1e | 42.2 ± 3.1e | 0.6 ± 0.1 | 0.8 ± 0.3 | ND |
| Hib-IIc | 4 | 27 ± 4e | 38 ± 3e | 34.4 ± 2.3e | 0.6 ± 0.1 | 1.6 ± 0.4 | ND |
a
Calculated as described in Materials and Methods.
b
Rats were immunized via IPP on day 0, received an i.t. boost on day 14, and were challenged with live bacteria on day 21. Serum and BAL samples were prepared as described in Materials and Methods.
c
Rats were immunized with killed bacteria from the strain of H. influenzae indicated and had received a live challenge with bacteria from the homologous strain.
d
ND, OMP26-specific antibody could not be detected at the lowest sample dilution.
e
P < 0.05 compared to nonimmune group.