Table 2.

Molecular diagnostic methods, primers, and target genes used for the detection and amplification of T. evansi.

Species	Name of the Assay	Primer Name	Oligonucleotide (5′-3′)	Gene Target	Reference
Trypanosoma spp.	PCR	CF BR	CCGGAAGTTCACCGATATTG TGCTGCGTTCTTCAACGAA	ITS1	[85]
Trypanozoon spp.	PCR	F R	ACATTCCAGCAGGAGTTGGAG CACGTGAATCCTCAATTTTGT	ESAG 6/7	[86]
Trypanozoon spp.	PCR	21mer 22mer	TGCAGACGACCTGACGCTACT CTCCTAGAAGCTTCGGTGTCCT	Repetitive sequence probe pMUTec 6.258	[87]
Trypanozoon spp.	PCR	TBR1 TBR2	GAATATTAAACAATGCGCAG CCATTTATTAGCTTTGTTGC	Minisatellite DNA	[88]
T. evansi and T. brucei	PCR	TBS-01 TBS-02	CGAATGAATAATAAACAATGCGCAGT AGAAGGATTTATTAGCTTTGTTGC	Conserved regions of T. brucei and T. evansi genome	[89]
T. evansi	PCR	TR3 TR4	GCGCGGATTCTTTGCAGACGA TGCAGACACTGGAATGTTACT	Repetitive nucleotide sequences of T. evansi	[90]
T. evansi	PCR	TeD-ISGF TeD-ISGR	CAGCCGGTGAGTGAAGAAA CTACGGCCCCTAATAATAAAGAAC	ISG-75	[91]
T. evansi	PCR	NRP1 NRP2	CGAATGAATATTAAACAATGCGCAGT AGAACCATTTATTAGCTTTGTTGC	Nuclid Repeat	[92]
T. evansi	PCR	MP1 MP2	CAACGACAAAGAGTCAGT ACGTGTTTTGTGTATGGT	Minicircle DNA	[92]
T. evansi	PCR	EVA1 EVA2	ACATATCAACAACGACAAAG CCCTAGTATCTCCAATGAAT	Minicircle DNA	[93]
T. evansi	PCR	DITRYF DITRYR	CGACCAGCCAGAACGAGCAGAAT CTTGTCGATCGAGTTGACGGT	VSG	[94]
T. evansi (Type A)	PCR	F R	GCGGGGTGTTTAAAGCAATA ATTAGTGCTGCGTGTGTTCG	Rotat 1.2 VSG	[17]
T. evansi (Type B)	PCR	F R	TTCTACCAACTGACGGAGCG TAGCTCCGGATGCATCGGT	VSG JN 2118Hu	[65]
T. evansi (Real-time PCR)	PCR	TeRoTat920F TeRoTat1070R	CTGAAGAGGTTGGAAATGGAGAAG GTTTCGGTGGTTCTGTTGTTGTTA	RoTat 1.2 VSG	[95]
T. evansi (Real-time PCR)	PCR	TeRTF TeRTR	GGAAGCAAAAGTCGTAACAAGG CCCATGTCAAACGGCATATAG	ITS1	[2]
T. evansi (TaqMan PCR)	PCR	F R Probe	ATAAATTGCACAGTATGCAACCAAA CATCCCTCATCTCCCATGTCA 6FAM-ACGGCATATAGAAACACA-MGBNFQ	ITS1 internal	[96]
T. evansi (Type A)	LAMP	F R F3 B3 FIP (F1c + F2) BIP (B1c + B2) LF LB	CAAAACTAACAGCCGTTGCAGCG AGTTCCGGTACCTTCTCCATTTC GTAGGAAGCAACACCTGCG TTGATTAGTGCTGCGTGTGT TGCGAGGTGCACCTTGATGTTGAAGCAATAACCGGCAACGAC GAAGGCAAAGTTGACGACCAGCTGTGGTGTGCTTTTCCTTGT GCGATTTTGATCCCGCCG CAGAACGAGCAGAATTTTCCA	Rotat 1.2 VSG	[22]
Trypanosoma spp.	LAMP	F3 B3 FIP (F1c + F2) BIP (B1c + B2) LF LB	CTGTCCGGTGATGTGGAAC CGTGCCTTCGTGAGAGTTTC GGAATACAGCAGATGGGGCGAGGCCAATTGGCATCTTTGGGA AAGGGAGACTCTGCCACAGTCGTCAGCCATCACCGTAGAGC GCCTCCCACCCTGGACTC AGACCGATAGCATCTCAG	RIME	[69]
T. evansi (Type A)	LAMP	F3 B3 FIP (F1c + F2) BIP (B1c + B2)	TCACAACAAGACTCGCACG GGGCTTTGATCTGCTCCTC TCAGAAGCGTCGAGCTGGGATTTTATCGACAATGCCATCGCC CGCAAGTTCCTGTGGCTGCATTTTTTCCCAAGAAGAGCCGTCT	PFR A1	[67]
T. evansi (Type B)	LAMP	F3 B3 FIP (F1c + F2) BIP (B1c + B2) LF LB	CCAATCAAAGACGAGCGG TGGTTTGTGAGGCCGCAG CGGATGCATCGGTGATGCAATCACTACTGCATCAAGGGAAGC ATCCAGCACCTCGGAACAGCTCTCGGCAACCAGATCGG GTTCACGTGCCTCCGCTTC ACGTAGCGGGAAAATACGC	VSG JN 2118Hu	[80]
T. evansi (Type A)	RPA	TevRPA-Fw TevRPA-Rv	CACCGAAGCAAGCGCAGCAAGAGGGTTAGCA GTAGCTGTCTCCTGGGGCCGAGGTGTCATAG	Rotat 1.2 VSG	[40]