Table 2.

Advanced Methods for Detection and Quantification of Resistant Starch.

Method Type	Technique	Principle and Application
Enzymatic	Megazyme Resistant Starch Assay	Employs a series of specific enzymes to simulate human digestion, followed by spectrophotometric quantification of the remaining RS. Widely used for its accuracy and reproducibility in diverse food matrices.
Chromatographic	High-Performance Liquid Chromatography (HPLC)	Involves enzymatic hydrolysis followed by HPLC analysis. This method distinguishes RS from other dietary fibers based on molecular size and structure, offering high precision and sensitivity.
Spectroscopic	Nuclear Magnetic Resonance (NMR) Spectroscopy	Utilizes advanced NMR techniques to provide molecular-level insights into RS structures. This method is instrumental in differentiating between RS types and understanding their digestion-resistant features.