Fig. 3.

On- and off-target effects identified by WGS analysis and undetected by Off-flow in CRISPR/Cas-edited clones. (A) IGV visualization. Deletions are displayed with a gray bar. The length of the bar indicates the size of the deletion. (B) Agarose gel electrophoresis of ∼700 bp PCR amplified fragment reveals a band of ∼400 bp from cell line CC-hom that was not present in other clones. (C) IGV visualization. Each color represents a different nucleotide. Red represents T and orange represents G. The bars are colored in proportion to the read count of each base. (D) Sequence trace showing the missense variant in CACNA1E in cell line HNDS0068–01 #B CC3.