Table 1.
List of CRISPR/Cas engineered iPSC lines.
| Gene | Locus (hg19) | Method | Position of target site (hg19) | Cell line ID |
|---|---|---|---|---|
| KCNQ2 | Chr20:62071001-62071003 (c.875_877delTCCinsCCT, p.L292_L293delinsPF) | Cas9-V3-Hifi RNP | Chr20:62,071,008-62,071,027 | HNDS0068-01 #B CNC14 HNDS0068-01 #B CC3 |
| KCNQ2 | Chr20:62073808 (c .766 G>T, p.G256W) | Cas9-V3-Hifi RNP | Chr20:62,073,830-62,073,848 | HNDS0078-01 #D CNC2 HNDS0078-01 #D CC8 HNDS0078-01 #D CC18 |
| KCNQ2 | chr20:62071057 (c .821 C>T, p.T274M) | Cas9-V3-Hifi RNP | Chr20:62,071,037-62,071,056 | HNDS0072-01 #C CNC87 HNDS0072-01 #C CC80 HNDS0072-01 #C CC20 |
| ASH1L | Chr1: 155451888 (c.773Gdel; p.G258fs) | Cas12a Ultra RNP | Chr1:155451881-155451901 | 1-1134-003_CNC5 1-1134-003_CC10 |
| ASH1L | Chr1: 155447758 (c.4902_4903TTdel; p.S1635fs) | Cas9-V3-Hifi RNP | Chr1:155,447,747-155,447,768 | 1-1217-003_CNC36 1-1217-003_CC37 |
| ASH1L | Chr1: 155450703 (c.1958dup; p.P654fs) | Cas12a Ultra RNP | Chr1:155,450,703-155,450,723 | 1-1006-003_CNC35 1-1006-003_CC9 |
| GNAQ | chr9:80412493 (c .548 G>A; p.R183Q) | Cas9 plasmid | chr9:80,412,499-80,412,518 | 31 CC-het 31 CC-hom |