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. 2023 Dec 29;23:638–647. doi: 10.1016/j.csbj.2023.12.036

Table 2.

List of in silico CRISPR off-target prediction tools and parameters used.

Tool Maximum mismatch number Maximum DNA bulge size
CHOPCHOP 3 n/a
Cas-OFFinder 5 2
CRISPRitz 5 2
CRISPR-offinder 5 n/a