Table 6.
gRNA and repair template sequences.
| Target locus (hg19) | Gene | sgRNA or crRNA | ssODN |
| Chr20:62071001-62071003 | KCNQ2 | ACCCCCAGACCTGGAACGGC (AGG) | AGCGCGAAGAAGGAGACACCGATGAGGGTGAAGGTTGCCGCAAGGAGCCTGCCTTTCCAAGTCTGGGGGTACTTGTCCCCGTAGCCAATGGTGGTCAGCGT |
| Chr20:62073808 | KCNQ2 | TCTCATCCTGGCCTCGTTCC (TGG) | GCCTGGTACATCGGCTTCCTTTGTCTCATCCTGGCCTCGTTCCTTGTATACTTGGCAGAGAAGGGGGAGAACGACCACTTTGACACCTACGCGGATGCACTCTGGTGGGGCCTGGTGAGTTGTGGTCA |
| Chr20:62071057 | KCNQ2 | GCTGACCACCATTGGCTACG (GGG) | GGGCGTCCAGCCTGCCCTCAGGGGTGTGAGCAGGCCCTTCGTGTGACTAGAGCCTGCGGTCCCACAGATCAcGtTGACCACCATTGGCTACGGGGACAAGTACCCCCAGACCTGGAACGGCAGGCTCCT |
| Chr1: 155451888 | ASH1L | ATCAGGAAAGCAGTGTTGGC (TTTG) | TGGCTTTTTTATTAAGTCCTTATGTATTATTCCAGCTACAGATCCAACACCTGCTTTCCGGATCAGATCCTTGCTAACCAATCCTGCTGTAGTGCCAGTT |
| Chr1: 155447758 | ASH1L | GTCCTGTGCAGAAGAGTCCA (GGG) | CGTTAGAAGGCAGTGACTCCTTTCTGTGAAGCCGATTTAGTGAAGTGTCTTGGGCAGAAAAGAGTCCAGGACTGTCAGTTAATTTCTTCCGGCCACTGGA |
| Chr1: 155450703 | ASH1L | CAAGGGAAGAGCTTATTCTT (TTTC) | GAATGCTGGATTCAGAAGTCAAACTTGGCTTCTTACCAAGGGAAGAGCTGATTCTTGGAATATCTATATGGGTAGTTTTTGAATCATTTACCTCTTTATC |
| Chr9:80412493 | GNAQ | CTAAGCACATCTTGTTGCGT (AGG) | n/a |
| ChrX:133620533-133620553 | HPRT1 | GATGATCTCTCAACTTTAAC (TGG) | n/a |
| ChrX:133609206-133609228 | HPRT1 | TGTAGGACTGAACGTCTTGCTCG (TTTC) | n/a |
| Nontarget (CRISPR-Cas9) | n/a | GCACTACCAGAGCTAACTCA | n/a |
| Nontarget (CRISPR-Cas12a) | n/a | CGTTAATCGCGTATAATACGG | n/a |