Figure 1.

Example of classical image registration problems. (a) Images acquired at different Z planes. The cartoons mimic images of cytoplasmic proteins into a cell acquired using a fluorescence microscope with the centre of the images dislocated at different X-Y positions. (b) Images of different signals from the same object. The cartoons mimic images of subcellular compartments acquired using the same fluorescence microscope with the centre of the images dislocated at different X-Y positions. (c) Images of the same object acquired using different acquisition modalities. The cartoons mimic images of the same cell acquired with a brightfield microscope and a fluorescent microscope. The centre of the images is dislocated at different X-Y positions.