Table 1.

Tools for registering multimodality 2D microscopy images—characteristics (X = available/yes; O = not available/no).

	AIBLROI	BigWarp	Correlia	ec-CLEM	elastix	ITK	LSAWSIFT	RVSS	StackReg	TrakEM2	DS4H-IA
VERSION
Year of first release	2006	2016	2020	2017	2010	1999	2008	2009	2010	2005	2022
Current version	O	7.0.5	1.0	1.0.1.5	5.0.1	5.2.1	28 October 2018	3.0.7	7 July 2011	1.3.6	1.0
DOCUMENTATION
User guide	X	X	X	X	X	X	O	X	X	X	X
Website	X	X	X	X	X	X	O	X	X	X	X
Video tutorial	O	X	O	X	X	X	O	O	O	X	X
Sample dataset	O	O	X	O	X	X	O	O	X	X	X
Open source	X	X	X	X	X	X	X	X	X	X	X
Implementation language	Java	Java	Java	Java	C++	C++	Java	Java	Java	Java	Java
USABILITY
Input image format	All common	All common	All common	All common	All common	All common	All common	All common	All common	All common	All common
No programming experience required	X	X	X	X	O	O	X	X	X	X	X
User-friendly GUI	X	X	O	O	O	O	O	X	X	O	X
Intuitive visualisation settings	X	O	O	O	O	O	O	O	X	O	X
No commercial licences required	X	X	X	X	X	X	X	X	X	X	X
Portability on Win/Linux/Mac	X	X	X	X	X	X	X	X	X	X	X
FUNCTIONALITY
Manual registration	X	X	X	X	O	X	O	O	X	X	X
Automatic registration	O	O	X	X	X	X	X	X	X	X	X
Image scale correction	X	X	X	X	X	X	O	X	X	X	X
Image rotation correction	X	X	X	X	X	X	O	X	X	X	X
Elastic correction	O	X	X	X	X	X	O	X	O	X	O
Multiple image handling	O	O	X	O	O	X	X	X	X	X	X
Multichannel/RGB image handling	O	X	X	X	X	X	X	X	X	X	X
OUTPUT
Resized aligned images	X	X	X	X	X	X	X	X	X	X	X
Full-sized aligned images	O	O	O	O	O	O	O	X	O	O	X
Registration parameters	O	X	X	X	X	X	O	X	X	X	X
Editable result	O	X	X	X	X	X	O	X	O	X	X