FIG. 3.

Localization of chlamydial hsp70 in normal, penicillin-exposed, and microbicide-exposed infected HEC-1B cells. Thin sections were probed with a polyclonal antibody generated against the carboxyl terminus of C. trachomatis hsp70 and labeled with a 30-nm-diameter gold-conjugated second-affinity antibody. Labeling of hsp70 in normally infected cells at 48 h p.i. (A), uninfected cells (control) (B), penicillin-induced persistently infected cells after 14 days (C), and hsp70 in infected cells exposed to C31G at 48 h p.i. (D) is shown. Magnifications: ×6,978 (A); ×8,723 (B); ×6,978 (C); and ×13,956 (D). h, translucent holes.