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Cytokine release by 50 and 200 nm INI-4001/A-SNP formulations from mBMDCs. mBMDCs (5 × 105/200 µL/well) were incubated with 50 and 200 nm INI-4001/A-SNP formulations overnight, and TNF-α, IL-6, and IL-12 release was determined by ELISA. A-SNP only, INI-4001 aqueous formulation in the same vehicle, and LPS served as controls. (A) Representative data of IL-6 releases by mBMDCs treated with 50 and 200 nm INI-4001/A-SNP. (B) MTT assay for different formulations.
