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Southern blot of total chromosomal DNAs from EHEC 86-24 (lanes 1), E. coli TUV86-2 (lanes 2), and the E. coli 86-24 exconjugant recombined with plasmid pTUV4 (lanes 3). The DNA was digested with either EcoRI (A) or DraI (B); a 1-kb DNA ladder served as the molecular size standard.
