TABLE 1.
List of oligonucleotide primers used in this study
| Primer | Sequence (5′ to 3′)a | Location (bp) | Reference |
|---|---|---|---|
| UB-3 | GC-TCT-AGA-CAG-AGC-AAT-TGC-CTT-CTG-AGC | 92–112 | 17 |
| Xbal | |||
| UB-2 | CG-GGA-TCC-GAC-GAC-TGA-TTT-GCA-TTC-CGG | 681–701 | 17 |
| BamHI | |||
| FG-1 | CG-GGA-TCC-GAG-TTT-TCC-AAG-TAT-AAT-GAG | 1,291–1,311 | 17 |
| BamHI | |||
| FG-2 | G-GAA-TTC-TTG-CCT-GGC-TCC-TCT-GGT-GAT | 870 downstream of FG-1 | This study |
| EcoRI | |||
| FG-5 | ATA-CCA-CTC-TGC-AAC-GTG | 645–662 | 17 |
| SLT-II-1 | CTT-CGG-TAT-CCT-ATT-CCC-GG | 288–307 | 27 |
| SLT-II-2 | GGA-TGC-ATC-TCT-GGT-CAT-TG | 747–766 | 27 |
a
Recognition sites for restriction endonucleases are underlined, and restriction endonucleases are indicated.