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The mouse phrenic nerve hemidiaphragm assay characterising the effects of rBoNT/A and rBoNT/A (0). (A) A schematic diagram showing the experimental setup of the preparation. (B) The effects of rBoNT/A (n = 3) and rBoNT/A (0) (n = 6) on hemidiaphragm contractility. rBoNT/A1 significantly and completely blocked the developed force after 20 min, which did not recover (p < 0.0001 2-way ANOVA).
