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. 2024 Jan 15;10(1):67. doi: 10.3390/jof10010067

Table 4.

Direct comparison between culture-dependent and culture-independent methods from 5 publications.

Source Culture-Dependent Method Culture-Independent Method Reference
No. of Isolates Phyla Name No. of Genera (Isolate) % Occurrence No. of OTUs Phyla Name % of Occurrence
Endophyte of aquatic plants 1689 3 Ascomycota 123 (1584) 93.8% 1074 6 Ascomycota 43.48% [68]
Basidiomycota 29 (92) 5.4% Basidiomycota 15.36%
Zygomycota 2 (13) 0.8% Zygomycota 1.49%
Chytridiomycota 1.21%
Glomeromycota 0.02%
Rozellomycota 0.01%
Unknown fungi 38.17%
Endophytes of Elymus repens 66 1 Ascomycota 9 (27) 40.9% 48 4 Ascomycota 90% [69]
Unidentified fungi Unknown (39) 59.1% Basidiomycota 2%
Glomeromycota 2%
Mortierellomycota 2%
Unknown fungi 4%
Endophytes of Vitis vinifera 94 1 Ascomycota 19 (94) 100% 59 3 Ascomycota 93.6% [70]
Basidiomycota 4.2%
Zygomycota 2.1%
Endophytes of Acanthus ilicifolius 203 2 Ascomycota 30 (200) 97.04% 111 2 Ascomycota 65.09% [14]
Basidiomycota 2 (3) 2.96% Basidiomycota 38.87%
Unknown fungal taxa 4.05%
Deep-sea sediment 19 2 Ascomycota 11 (14) 73.7% 42 2 Ascomycota 59.5% [71]
Basidiomycota 2 (5) 26.3 Basidiomycota 40.5%