TABLE 1.
Tryptophan catabolism in four genital tract cell lines
| Cell line | Tissue origin | IFN-γ treatmenta | IDO activityb (% specific catabolism ± SD)
|
|
|---|---|---|---|---|
| Nonpolarized | Polarized | |||
| Me180 | Cervical epithelium | − | 0 | 0 |
| + | 65.2 ± 13.0 | 58.2 ± 3.2 | ||
| HeLa | Cervical epithelium | − | 0 | ND |
| + | 61.0 ± 12.1 | ND | ||
| Ishikawa | Endometrial epitheliumc | − | 1.2 ± 1.8 | ND |
| + | 1.3 ± 1.5 | ND | ||
| Hec1B | Endometrial epithelium | − | 0 | ND |
| + | 0 | ND | ||
a
+, treated cells; −, untreated cells. Cells were treated with 10 ng of IFN-γ/ml for 48 h before being pulsed with [3H]tryptophan in Hanks balanced salt solution for 4 h.
b
Values are the mean results for at least two independent experiments in which samples were assayed in duplicate or triplicate. Me180 was the only cell line polarized in this study. ND, not determined.
c
Hormone responsive.