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. 2024 Jan 4;151(1):dev201819. doi: 10.1242/dev.201819

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© 2024. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 1. — Tubb4 immunofluorescence staining of multi-ciliated cells. (A-C) Immunofluorescent staining of respiratory MCCs at (A) P1 and (B,C) P5. Tubb4 signal is enriched in distal-most 57±10.7% of axonemes (mean±s.d., n=85 cells from six animals). (D-F) Immunofluorescent staining of oviduct epithelium in adults. Tubb4 signal is enriched in distal 54.4±9.8% of axonemes (n=36 cells from two animals). Scale bars: 5 µm in A-E; 10 µm in F. (G) Tubb4 staining of adult ependymal cells. Scale bar: 10 µm. Arrow indicates MCC cilia; arrowheads indicate primary cilia.