TABLE 3.
Proteolytic activity of anthrax LF mutants
| Enzyme | % Cleavage of peptide 1a |
|---|---|
| LF (wt) | 85.9 |
| LF (recombinant) | 82.1 |
| LFH686A | 3.4 |
| LFH690A | 3.2 |
| LFH686A+H690A | 3.0 |
| LFE687C | 2.4 |
| LFE687D | 0 |
| BSAb | 0 |
| Trypsin | 100 |
| Pronase | 100 |
a
A 500 μM concentration of peptide 1 (ELYENKPRRPYIL) was mixed with 23 ng (250 nM) of enzyme and allowed to react for 18 h at 37°C. Samples were analyzed on an HPLC as described in Materials and Methods. The areas of the substrate and products peaks were measured and compared to that of substrate controls (no enzyme). The results are averages of duplicate experiments.
b
BSA, bovine serum albumin.