Figure 2.

Immunoreactive Ki-67⁺CD25⁺ cell populations increase over time upon MLR stimulation. (A, B) Flow cytometry UMAP dimensionality reduction representation of CD4⁺ (A) and CD8⁺ (B) T cells upon MLR stimulation colored by non-supervised clustering. (C, D) Barplot of cell proportions colored by non-supervised clustering for CD4⁺ (C) or CD8⁺ T cells. (E, F) Barplot of Ki-67 and CD25 expression upon CD4 (E) or CD8 (F) MLRs. (G) Spearman correlation between IFN-γ secretion and percentage of Ki-67⁺CD25⁺ in CD4⁺ T cells. (H) Spearman correlation between granzyme B secretion and percentage of Ki-67⁺CD25⁺ in CD8⁺ T cells. TCR stim: T cells stimulated with anti-CD3 and anti-CD28 antibodies.