FIG. 2.

mRNA transcripts for human IL-1β, IL-8, and human TNF-α are induced when human intestinal xenografts are infected with C. parvum. The results of an RT-PCR assay for mRNA transcripts for human IL-1β, IL-8, human TNF-α, and actin from tissue samples from an intestinal xenograft infected 7 days previously with C. parvum (lanes +) and the control uninfected contralateral graft (lanes −) are shown. Transcripts of the predicted sizes for human IL-1β (339 bp), IL-8 (281 bp), and human TNF-α (610 bp) are amplified in the C. parvum-infected graft but are not detectable in control xenografts. The equivalent density of the actin control suggests that equivalent quantities of cDNA were present in the samples. Lane φX contains the φX174RF/HaeIII DNA size standards.