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Cathepsin D in B. abortus vacuoles analyzed by double immunofluorescence. HeLa cells were immunostained to localize both Brucella (A and C) and cathepsin D (B and D). Double immunofluorescence micrographs show that strain 2308 does not colocalize with cathepsin D (A and B), whereas in strain 19-infected cells few remaining bacteria or bacterial degradation products colocalize with the lysosomal marker (C and D) as indicated by arrows. Bar, 10 μm.
