Table 1.
Analytical sensitivity (LoD) in undiluted and diluted urine.
| TCID50 | Undiluted Urine (Mean Ct ± SD 3) |
Undiluted Urine Hit Score |
Diluted Urine (Mean Ct ± SD 3) |
Diluted Urine Hit Score |
Undiluted-Diluted Mean Ct (ΔCt) |
|---|---|---|---|---|---|
| 106 | 29.57 (±0.25) | 4/4 | 21.20 (±0.09) | 4/4 | 8.37 |
| 105 | 32.54 (±0.35) | 4/4 | 24.59 (±0.04) | 4/4 | 7.95 |
| 104 | 35.10 1 (±0.36) | 4/4 | 27.93 (±0.20) | 4/4 | 7.17 |
| 103 | Nd 2 | 0/4 | 31.28 (±0.11) | 4/4 | |
| 102 | nd | 0/4 | 34.54 (±0.20) | 4/4 | |
| 101 | nd | 0/4 | 37.87 1 (±0.69) | 4/4 | |
| 1 | nd | 0/4 | 40.81 (±1.01) | 3/4 | |
| 0.1 | nd | 0/4 | nd | 0/4 | |
| 0.01 | nd | 0/4 | nd | 0/4 | |
| 0.001 | nd | 0/4 | nd | 0/4 |
1 Limit of detection (LoD); 2 nd: not detected; 3 SD: standard deviation.