TABLE 4.
Proportions and phenotypes of blast cells following in vitro culture for 7 days with or without rIL-2 (n = 5)
| Culture | % of cells in R2 | % of CD3+ blasts (mean ± SEM) expressing:
|
|||
|---|---|---|---|---|---|
| CD4 | CD8 | TCRαβ | TCRγδ | ||
| uRBC | 3.0 ± 0.7 | 57.9 ± 2.6 | 34.3 ± 2.4 | 92.2 ± 1.7 | 7.8 ± 1.7 |
| uRBC with IL-2 | 29.2 ± 11.9 | 37.9 ± 9.0 | 32.7 ± 5.3 | 70.6 ± 6.1 | 29.4 ± 6.1 |
| Paired t (P [df = 4]) | 2.15 (0.1) | 2.11 (>0.1) | 0.24 (>0.5) | 3.99 (<0.02) | 3.99 (<0.02) |
| PfSE | 12.0 ± 1.52 | 74.6 ± 3.69 | 18.8 ± 2.62 | 93.4 ± 1.29 | 6.6 ± 1.29 |
| PfSE with IL-2 | 42.0 ± 10.9 | 38.1 ± 10.8 | 21.3 ± 2.60 | 59.4 ± 10.85 | 40.6 ± 10.85 |
| Paired t (P [df = 4]) | 2.66 (>0.05) | 3.76 (<0.02) | 1.36 (>0.2) | 3.41 (<0.03) | 3.41 (<0.03) |
| Live P. falciparum | 20.0 ± 7.40 | 29.3 ± 4.57 | 22.1 ± 2.23 | 51.4 ± 4.02 | 48.6 ± 4.02 |
| Live P. falciparum with IL-2 | 27.0 ± 5.52 | 40.7 ± 6.70 | 20.7 ± 1.58 | 61.4 ± 5.65 | 38.6 ± 5.65 |
| Paired t (P [df = 8]) | 1.50 (>0.1) | 1.80 (>0.1) | 0.61 (>0.5) | 1.58 (>0.1) | 1.58 (>0.1) |