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Protein analysis of AFPE by SDS-PAGE: Three different AFPE preparations (1, 2, and 3) were loaded (10 and 15 μL) onto SDS-PAGE, which was carried out using 15% polyacrylamide separating gel, and stained with Coomassie R-250 blue. M, molecular weight marker; the arrow shows the ~33 kDa protein.
