Fig. 2.
Global depletion of myeloid IKKβ results in reduced axonal damage and oxidative stress in EAE. A Specimen images of paraffin-embedded coronal spinal cord sections from IKKβF/F (upper panel) and ΜφΙΚΚβKO (lower panel) mice with MOG35-55-induced EAE at 23 dpi (chronic phase). Serial sections were stained for markers of inflammation and demyelination, such as hematoxylin and eosin (H&E) and Luxol fast blue (LFB), respectively. Representative images from immunohistochemistry for markers of axonal damage (APP and Biel. Spheroids), immune cell infiltration (CD3 and Mac3), myeloid cell activation (iNOS) and macrophagic oxidative stress (p22 phox) are also shown. B Semi-quantitative scoring of immune cell infiltration (increased intensity of H/E staining) and demyelination (loss of LFB staining) of the same groups of mice shown in A. C Quantification index of axonal damage measured as levels of axonal APP and number of Biel. Spheroids of the same groups of mice shown in A. D Quantification index of myeloid cell activation measured as levels of iNOS and number of macrophagic p22 phox of the same groups of mice shown in A. Numbers of mice are annotated as scatter dots on the bars. All mice were adult females 2–4 months old