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. 2023 Dec 13;300(1):105562. doi: 10.1016/j.jbc.2023.105562

Figure 10.

Figure 10

Effects of HDAC1/2 and USP7 on the development of yak embryos.A and B, expression analysis of HDAC1/2 in different tissues of yak. C, expression analysis of USP7 in different tissues of yak. D, expression profile of USP7 in preimplantation embryonic development in the yak. E, representative photos in the bright fields on day 5 and day 7 after fertilization of yak embryos treated with DMSO or FK228. The scale bar represents 100 μm. F and G, scatter plots show the total cell number, ICM cell number, and TE cell number of blastocysts in the FK228-treated and P5091-treated groups and the corresponding control groups, respectively. H and I, immunofluorescence (IF) staining of CDX2 in yak blastocysts after DMSO or FK228 treatment. The scale bar represents 50 μm. J and K, IF of CDX2 in yak blastocysts after DMSO or P5091 treatment. The scale bar represents 50 μm. L and M, IF of 5 mC and 5hmC in yak blastocysts after DMSO or P5091 treatment. The scale bar represents 50 μm. (n = 3; 10–20 embryos per group per replicate). Data shown as Means ± SEM. ∗ p < 0.05, ∗∗ p < 0.01. DMSO, dimethyl sulfoxide; HDAC, histone deacetylases; ICM, inner cell mass; TE, trophectoderm.