. Author manuscript; available in PMC: 2024 Jan 27.

Published in final edited form as: J Am Chem Soc. 2023 Apr 19;145(17):9417–9422. doi: 10.1021/jacs.3c01032

Table 1.

Dynamic Range (DR) and Relative Quantum Yield (QY) for FAM-Labeled Oligos.^a

Construct	DR (fold)	QY_a/QY_a0	QY_b/QY_b0
5′-FAM-U₅-3′	13.0	0.55	0.94
5′-FAM-U₄G-3′	15.6	0.38	0.77
5′-FAM-GU₄-3′	18.1	0.13	0.33
5′-FAM-G₂U₃-3′	30.7	0.06	0.28
Fluorescein	15.4	1.00	1.00
Probe	55.9	N/A	N/A
Probe-DNA	48.9	N/A	N/A
Probe-RNA	30.3	N/A	N/A

Underlined bases signify 2′-OMe RNA. QY_a and QY_a0 refer to sample and free dye quantum yields, respectively, at pH 4.9. QY_b and QY_b0 are measured at pH 8.0.