TABLE 1.
Shigella species | Treatment | Avg no. of log10 viable bacteria ± SEa
|
|||
---|---|---|---|---|---|
Not centrifuged
|
Centrifuged
|
||||
Control | Cytochalasin | Control | Cytochalasin | ||
S. sonnei | Cytochalasin | 1.9 ± 0.1 | 2.0 ± 0.1 | 3.9 ± 0.2 | 3.2 ± 0.2b |
Calcium-free medium | 2.3 ± 0.2 | 3.6 ± 0.1c | 3.8 ± 0.2 | 4.6 ± 0.1c | |
S. flexneri | Cytochalasin | 1.8 ± 0.1 | 2.0 ± 0.1 | 2.7 ± 0.1 | 2.9 ± 0.1 |
Calcium-free medium | 1.8 ± 0.1 | 3.6 ± 0.1c | 2.8 ± 0.2 | 4.7 ± 0.1c |
Average of at least three separate assays, with a lower detection limit of 50 bacteria (1.7 log10). Numbers of internalized shigellae consistently increased in control enterocytes that were centrifuged compared to control enterocytes that were not centrifuged (P < 0.01).
Significantly decreased compared to corresponding control enterocytes, P < 0.05.
Significantly increased compared to corresponding control enterocytes, P < 0.01.