TABLE 2.
Decrease in TEER of mature Caco-2 cultures following 1 h of incubation in calcium-free medium or in tissue culture medium supplemented with either cytochalasin or colchicine
| Treatment | % Decrease in TEER (avg ± SE of ≥4 cultures) |
|---|---|
| None | 5 ± 1 |
| Colchicine, 25 μg/ml | 12 ± 2 |
| Cytochalasin | |
| 0.1 μg/ml | 27 ± 2a |
| 1.0 μg/ml | 68 ± 3a |
| 10 μg/ml | 51 ± 3a |
| Calcium-free medium | 94 ± 1a |
a
Significant decrease in TEER compared to no treatment, analysis of variance followed by Fisher’s test for significant difference, P < 0.01.