Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2024 Jan 27;15:828. doi: 10.1038/s41467-023-44348-y

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 4 — a Volcano plot showing differentially expressed genes in CR+LSD1i and CR APL blasts. Genes in red/blue are significantly up/down regulated (FDR < 0.05). Relevant ISGs are highlighted. b Top hallmark gene sets (from MSigDB) enriched in SD, CR and OSI-906 APLs treated with LSD1 inhibitor. The color of the dot indicates the normalized enrichment scores (NES) and size of the dot indicates the FDR q values. Only dots with an FDR < 0.25 are reported. c Heatmap of log2 fold-change regulation in dsRNA-sensing and ISGs genes relative to SD untreated APLs. The color scale indicates the log2 fold-change. d Linear regression analysis of fold change regulation in ERV expression showing LSD1-inhibition - dependent de-repression of dsRNA-inducing ERVs in CR. e UMAP visualization of scRNAseq data from of 18,020 cells grouped in 19 clusters. Cells are colored by clusters. f Hierarchical clustering highlighting the first bifurcation on Cd177 positivity: stem-like clusters 15-17 vs all other non-stem clusters. g UMAP visualization of cells split by treatment conditions (SD, CR, SD+LSD1i, and CR+LSD1i, as indicated). Cd177 + Cd34+ cells are highlighted in red and their frequency per condition is indicated. h Average log2 fold change (scLIC vs non-scLIC) in genes belonging to the indicated Hallmark gene sets in the scRNAseq data, by treatment condition. i Expression of Gfi1 and Irf8 within the scLIC (Cd34 + /Cd177hi) and non-scLIC (non-Cd34 + /Cd177hi) cells in the scRNAseq. Left panel shows expression levels (normalized counts), right panels show the corresponding fraction of cells (expressed as percentage of normal/gated). j GSEA plots showing enrichment of TRAIL sensitivity signature upon DDP treatment in CR and SD samples. NES, normalized enrichment score. Source data are provided as a Source Data file.