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. 2024 Jan 27;15:818. doi: 10.1038/s41467-024-45097-2

Fig. 5. Intrinsic alteration of immune program in oncogenic acinar and ductal cells.

Fig. 5

a Expression heatmap of genes in the GOBP antigen processing and presentation gene set in fresh, metaplastic, and KPTS cells. K means clustering was performed to identify different expression patterns across all groups of samples. b Schematic illustration of key gene changes in antigen processing and presentation pathways in cultured acinar and ductal cells. c Boxplot of RNA expression of MHC class I and PD-L1 (CD274) genes in all groups of samples. The middle line of box represents the median value, the bounds of box represent the IQR, and the whiskers extend to 1.5 × IQR. Fresh ductal n = 5, fresh acinar n = 5, acinar-metaplastic n = 11, acinar-KPTS n = 3, ductal-KPTS n = 4. d Left: representative flow cytometry analysis of MHC I molecule and mouse IgG isotype control from 3 independent paired metaplastic and KPTS acinar organoids. Right: quantification of MHC I mean fluorescence from 3 independent paired metaplastic and KPTS samples. The fluorescence intensity of MHC I in each sample was adjusted by subtracting the intensity of corresponding isotype control, before analyzed by two-tailed Student’s t test. Error bar represents standard deviation. e Expression heatmap of genes in the KEGG complement and coagulation cascade gene set in fresh, metaplastic, and KPTS cells.