Fig. 2.

Tumor locales with high HSF1 expression were inhabited by macrophages. A, B Normalized relative expression of Hsp70 (A) and Ki67 (B) in tumors from mice after 32 days of treatment; *p < 0.05; **p < 0.001. C Representative immunostaining fluorescence images of A549 tumor slices from four experimental groups with inserts. pHSF1^Ser326 was stained in red, the nucleus was stained with DAPI in blue. D Counting of A549 cells expressing HSF1 in tumor slices from four experimental groups. Tumor slices from three mice from each group were counted. Data are presented as a percentage of all tumor cells. E Slices from the ‘Contr’ group were stained with anti- pHSF1^Ser326 antibody (red) and with an antibody to macrophage marker Iba1 (green); the nucleus was stained with DAPI (blue)