Fig. 3.

Co-cultivation of tumor cells with human monocytes or monocyte-like THP1 cells led to an increase in HSF1 activity in tumor cells. A Western blot analysis of A549 and DLD1 cells after incubation either with THP1 cells of with human monocytes for 20 h performed with pHSF1^Ser326 and HSP70 antibodies. β-tubulin was used as the loading control. B HSE-luciferase reporter assay performed with HeLa_luc cells incubated with 5 µM U133, a well-known activator of HSF1, 20 h after the addition of U133. Data are presented in arbitrary luminescence units; C HSE-luciferase reporter assay of cells after co-cultivation with THP1 or human mononcytes. Cells were analyzed 20 h after the addition of THP1 or human monocytes. D A549 cells were seeded on E-plate and proliferation activity was measured using xCELLigence. THP1 cells were added after 20 h onto special inserts which contains a 0.4 μm pore size membrane. Etoposide was added 20 h after the addition of THP1