Fig. 5.

Monocytes reduce the sensitivity of HCC cells to cytotoxic agents. A HCC cells were preincubated with human monocytes for 20 h and then either etoposide was added for 36 h (left panel) or co-cultivated with THP1 after 20 h. After that, CQ was administered for 24 h (right panel). The apoptosis was measured with the aid of flow cytometry with use of Annexin V Alexa 647 and propidium iodide (PI). Each panel shows representative data from one of three independent experiments. B HCC cells were treated as described above. The cell index was measured using the xCELLigence system for 35–40 h