Fig. 6.

In cells with HSF1 knock-down and co-incubation with monocyte-like cells, resistance to etoposide does not form. A Western blot analysis of the level of total HSF1 (tHSF1) and active pHSF1^Ser326 in A549_scr ( A549 cells, transfected with empty plasmid) or A549_kdHSF1 (∆HSF1) cells after co-cultivation with THP1for 20 h. β-tubulin was used as the loading control. B Spheroids were formed from A549scr or A549_kdHSF1 cells with or without incubation with THP1 cells. After 6 days, etoposide was added at a concentration of 200 μm. Images were taken at 60 h. C Spheroids were treated as described in (B) and viability analyzed using CellTiter-Glo 3D kit (Promega, US). *p < 0.05; **p < 0.001